Volume 42, Issue 8 p. 961-968

Flavour development via lipolysis of milkfats: changes in free fatty acid pool

Mafalda A. Regado

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P‐4200–072 Porto, Portugal

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Betina M. Cristóvão

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P‐4200–072 Porto, Portugal

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Carla G. Moutinho

Centro de Bioengenharia e FármacoClínica, Universidade Fernando Pessoa, Praça 9 de Abril, P‐4249–004 Porto, Portugal

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Victor M. Balcão

Centro de Bioengenharia e FármacoClínica, Universidade Fernando Pessoa, Praça 9 de Abril, P‐4249–004 Porto, Portugal

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Raquel Aires‐Barros

Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Avenida Rovisco Pais, P‐1049–001 Lisboa, Portugal

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João Paulo M. Ferreira

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P‐4200–072 Porto, Portugal

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F. Xavier Malcata

Corresponding Author

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P‐4200–072 Porto, Portugal

*Fax: +351 22 5090351; e‐mail: fxmalcata@esb.ucp.ptSearch for more papers by this author
First published: 10 May 2007
Citations: 22

Summary

Under the trade name lipolysed milkfat (LMF), concentrated cheese flavours obtained via enzymatic treatments of cheese (or other dairy substrates) have been made available in increasing numbers. In this research effort, (anhydrous) milkfats from cows, ewes and goats were used as substrates for production of cheesy flavours. Those milkfats were subjected to modifications brought about by ten different (commercial) lipases and one cutinase, and the free fatty acids released were analysed by high performance liquid chromatography. Both the degrees of hydrolysis and the free fatty acid profiles of the final products were similar to those reported for several LMF products, although specific keynotes could be pinpointed. Consequently, those milkfats may represent alternative raw materials for manufacture of cheesy flavours, while contributing to alleviate the problem derived from the increasing surplus of milkfat in Western countries.