Original Article
Antioxidant activity of blueberry anthocyanin extracts and their protective effects against acrylamide-induced toxicity in HepG2 cells
Xuenan Li,
Huangyou Liu,
Lingzhu Lv,
Haiyang Yan,
Yuan Yuan,
Xuenan Li
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorHuangyou Liu
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorLingzhu Lv
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorHaiyang Yan
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorCorresponding Author
Yuan Yuan
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Correspondent: E-mail: [email protected]Search for more papers by this authorXuenan Li,
Huangyou Liu,
Lingzhu Lv,
Haiyang Yan,
Yuan Yuan,
Xuenan Li
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorHuangyou Liu
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorLingzhu Lv
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorHaiyang Yan
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Search for more papers by this authorCorresponding Author
Yuan Yuan
College of Food Science and Engineering, Jilin University, Changchun, 130062 China
Correspondent: E-mail: [email protected]Search for more papers by this authorXuenan Li and Huangyou Liu are the joint first author.
Summary
In this study, the antioxidant activities of blueberry anthocyanin extracts from ten blueberry varieties were evaluated based on the methods of scavenging activities for DPPH radicals, ABTS radicals, hydroxyl radicals and ferric reducing antioxidant power (FRAP) assay. Among the ten blueberry varieties, Polaris had the highest antioxidant abilities and the largest amounts of anthocyanins identified by HPLC-MS. The protective effects of anthocyanin extracts from Polaris (AEP) against acrylamide (AA)-induced toxicity in HepG2 cell models were also evaluated due to the neurotoxic, genotoxic and potentially carcinogenic effects of AA. The protective effects of AEP on the damage of HepG2 cells were explored from the aspects of cell viability, T-SOD and CAT activity and MDA level. The AEP (5, 10, 20 μg mL−1) could significantly increase cell viability (P < 0.01) and inhibit AA-induced cytotoxicity. Polaris also markedly promoted the activity of SOD, CAT and inhibited MDA level. The results showed that AEP had strong antioxidant activities, presenting high protective effects against AA-induced cell damage in HepG2 cell models.
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