Steviol glycosides, sweet diterpenes extracted from the shrub Stevia rebaudiana Bertoni, are approved as sweeteners in many countries throughout the world. Former heat and pH-investigations of these glycosides have established their stability. However, due to the complex purification process, the natural authenticity has still been discussed and challenged, recently. Thus, the objective of this work was to show that the steviol glycosides are not chemically modified during the commercial extraction and purification process. Therefore, samples of three independent commercial-scale extraction and purification batches of steviol glycosides, each batch containing a sample of the untreated stevia leaves, the first water extract and the high-purity end product, were analysed using HPLC-UV and HPLC-ESI-MS/MS. The results show that the commercial powders of extracted steviol glycosides with an estimated purity of more than or equal to 95% contain the same steviol glycosides as the dried stevia leaves and their hot water infusions, demonstrating that steviol glycosides are not affected by the purification process.
|ijfs13494-sup-0001-FigS1.docxWord document, 142.8 KB||Figure S1. Chromatograms of the USP-standard using RP- (A) and HILIC-separation (B)|
|ijfs13494-sup-0002-FigS2.docxWord document, 140.7 KB||Figure S2. Chromatograms of SL- (A), ESL- (B) and SLE95 (C)-samples using RP-separation|
|ijfs13494-sup-0003-FigS3.docxWord document, 140.7 KB||Figure S3. Chromatograms of ESL- (A) and SLE95 (B)-samples, separated via HILIC.|
|ijfs13494-sup-0004-TableS1.docxWord document, 27.6 KB||Table S1. Mean values of peak areas in percentage [%] with the standard deviation of the eight steviol glycosides identified in SL, ESL and SLE95 samples of each production batch after RP-separation.|
|ijfs13494-sup-0005-TableS2.docxWord document, 17.5 KB||Table S2. Retention times of the nine steviol glycosides according to the USP reference standard and samples using RP- and HILIC-conditions.|
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